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äkta prime plus fplc system  (GE Healthcare)
96
GE Healthcare äkta prime plus fplc system
(A) Chromatographic profile of Bps Omp38 purification with a Hitrap SP HP column, connected to an <t>ÄKTA</t> Prime plus <t>FPLC</t> system. The protein was eluted with a linear gradient of 0–1 M KCl, (B) SDS/PAGE followed by Coomassie Blue staining, (C) immunoblot analysis of the same samples as in panel B, using polyclonal anti- Bps Omp38 serum, (D) immunoblot analysis using anti-His 6 monoclonal antibody, and (E) identification of tryptic digests of the expressed proteins by nanoLC/ESI MS (First BASE Laboratories, Malaysia). Eight peptides, designated P1–P8 and shown in red, were unambiguously identified by MASCOT as identical to internal sequences of Bps Omp38. The 20-aminoacid signal peptide is underlined. Lanes: Std, standard proteins; 1, Bps Omp38WT; 2, Bps Omp38Y119A; 3, Bps Omp38 mY119F; 4, refolded Bps Omp38; 5, E. coli OmpF; 6, E. coli OmpN; 7, and V. harveyi ChiP.
äkta Prime Plus Fplc System, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/äkta prime plus fplc system/product/GE Healthcare
Average 96 stars, based on 1 article reviews
äkta prime plus fplc system - by Bioz Stars, 2026-05
96/100 stars
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biologic duoflow system fast protein liquid chromatography instrument  (Bio-Rad)
96
Bio-Rad biologic duoflow system fast protein liquid chromatography instrument
(A) Chromatographic profile of Bps Omp38 purification with a Hitrap SP HP column, connected to an <t>ÄKTA</t> Prime plus <t>FPLC</t> system. The protein was eluted with a linear gradient of 0–1 M KCl, (B) SDS/PAGE followed by Coomassie Blue staining, (C) immunoblot analysis of the same samples as in panel B, using polyclonal anti- Bps Omp38 serum, (D) immunoblot analysis using anti-His 6 monoclonal antibody, and (E) identification of tryptic digests of the expressed proteins by nanoLC/ESI MS (First BASE Laboratories, Malaysia). Eight peptides, designated P1–P8 and shown in red, were unambiguously identified by MASCOT as identical to internal sequences of Bps Omp38. The 20-aminoacid signal peptide is underlined. Lanes: Std, standard proteins; 1, Bps Omp38WT; 2, Bps Omp38Y119A; 3, Bps Omp38 mY119F; 4, refolded Bps Omp38; 5, E. coli OmpF; 6, E. coli OmpN; 7, and V. harveyi ChiP.
Biologic Duoflow System Fast Protein Liquid Chromatography Instrument, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biologic duoflow system fast protein liquid chromatography instrument/product/Bio-Rad
Average 96 stars, based on 1 article reviews
biologic duoflow system fast protein liquid chromatography instrument - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
amersham fplc system äkta device  (Amersham Life Sciences Inc)
86
Amersham Life Sciences Inc amersham fplc system äkta device
(A) Chromatographic profile of Bps Omp38 purification with a Hitrap SP HP column, connected to an <t>ÄKTA</t> Prime plus <t>FPLC</t> system. The protein was eluted with a linear gradient of 0–1 M KCl, (B) SDS/PAGE followed by Coomassie Blue staining, (C) immunoblot analysis of the same samples as in panel B, using polyclonal anti- Bps Omp38 serum, (D) immunoblot analysis using anti-His 6 monoclonal antibody, and (E) identification of tryptic digests of the expressed proteins by nanoLC/ESI MS (First BASE Laboratories, Malaysia). Eight peptides, designated P1–P8 and shown in red, were unambiguously identified by MASCOT as identical to internal sequences of Bps Omp38. The 20-aminoacid signal peptide is underlined. Lanes: Std, standard proteins; 1, Bps Omp38WT; 2, Bps Omp38Y119A; 3, Bps Omp38 mY119F; 4, refolded Bps Omp38; 5, E. coli OmpF; 6, E. coli OmpN; 7, and V. harveyi ChiP.
Amersham Fplc System äkta Device, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amersham fplc system äkta device/product/Amersham Life Sciences Inc
Average 86 stars, based on 1 article reviews
amersham fplc system äkta device - by Bioz Stars, 2026-05
86/100 stars
  Buy from Supplier
äkta fast protein liquid chromatography instrument  (Amersham Life Sciences Inc)
86
Amersham Life Sciences Inc äkta fast protein liquid chromatography instrument
(A) Chromatographic profile of Bps Omp38 purification with a Hitrap SP HP column, connected to an <t>ÄKTA</t> Prime plus <t>FPLC</t> system. The protein was eluted with a linear gradient of 0–1 M KCl, (B) SDS/PAGE followed by Coomassie Blue staining, (C) immunoblot analysis of the same samples as in panel B, using polyclonal anti- Bps Omp38 serum, (D) immunoblot analysis using anti-His 6 monoclonal antibody, and (E) identification of tryptic digests of the expressed proteins by nanoLC/ESI MS (First BASE Laboratories, Malaysia). Eight peptides, designated P1–P8 and shown in red, were unambiguously identified by MASCOT as identical to internal sequences of Bps Omp38. The 20-aminoacid signal peptide is underlined. Lanes: Std, standard proteins; 1, Bps Omp38WT; 2, Bps Omp38Y119A; 3, Bps Omp38 mY119F; 4, refolded Bps Omp38; 5, E. coli OmpF; 6, E. coli OmpN; 7, and V. harveyi ChiP.
äkta Fast Protein Liquid Chromatography Instrument, supplied by Amersham Life Sciences Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/äkta fast protein liquid chromatography instrument/product/Amersham Life Sciences Inc
Average 86 stars, based on 1 article reviews
äkta fast protein liquid chromatography instrument - by Bioz Stars, 2026-05
86/100 stars
  Buy from Supplier

Image Search Results


(A) Chromatographic profile of Bps Omp38 purification with a Hitrap SP HP column, connected to an ÄKTA Prime plus FPLC system. The protein was eluted with a linear gradient of 0–1 M KCl, (B) SDS/PAGE followed by Coomassie Blue staining, (C) immunoblot analysis of the same samples as in panel B, using polyclonal anti- Bps Omp38 serum, (D) immunoblot analysis using anti-His 6 monoclonal antibody, and (E) identification of tryptic digests of the expressed proteins by nanoLC/ESI MS (First BASE Laboratories, Malaysia). Eight peptides, designated P1–P8 and shown in red, were unambiguously identified by MASCOT as identical to internal sequences of Bps Omp38. The 20-aminoacid signal peptide is underlined. Lanes: Std, standard proteins; 1, Bps Omp38WT; 2, Bps Omp38Y119A; 3, Bps Omp38 mY119F; 4, refolded Bps Omp38; 5, E. coli OmpF; 6, E. coli OmpN; 7, and V. harveyi ChiP.

Journal: PLoS ONE

Article Title: Porin Involvement in Cephalosporin and Carbapenem Resistance of Burkholderia pseudomallei

doi: 10.1371/journal.pone.0095918

Figure Lengend Snippet: (A) Chromatographic profile of Bps Omp38 purification with a Hitrap SP HP column, connected to an ÄKTA Prime plus FPLC system. The protein was eluted with a linear gradient of 0–1 M KCl, (B) SDS/PAGE followed by Coomassie Blue staining, (C) immunoblot analysis of the same samples as in panel B, using polyclonal anti- Bps Omp38 serum, (D) immunoblot analysis using anti-His 6 monoclonal antibody, and (E) identification of tryptic digests of the expressed proteins by nanoLC/ESI MS (First BASE Laboratories, Malaysia). Eight peptides, designated P1–P8 and shown in red, were unambiguously identified by MASCOT as identical to internal sequences of Bps Omp38. The 20-aminoacid signal peptide is underlined. Lanes: Std, standard proteins; 1, Bps Omp38WT; 2, Bps Omp38Y119A; 3, Bps Omp38 mY119F; 4, refolded Bps Omp38; 5, E. coli OmpF; 6, E. coli OmpN; 7, and V. harveyi ChiP.

Article Snippet: Bps Omp38 was further purified by cation exchange chromatography using a Hitrap SP HP column (5×1 mL) connected to an ÄKTA Prime plus FPLC system (GE Healthcare Life Sciences, Life Sciences Instruments, ITS (Thailand) Co., Ltd., Bangkok, Thailand).

Techniques: Purification, SDS Page, Staining, Western Blot